Mazukora On the other hand our results suggest that the major MVA deletions contribute to attenuation of this virus, but they cannot explain why high MVA doses are needed to achieve maximum vaccine potency. The vaccinia virus VACV Lister strain was one of the vaccine strains that enabled smallpox eradication. The influence of deleting region VI was not investigated because it was thought unlikely to affect virulence since it encompasses one gene fragmented in all VACV strains an orthologue of the CPXV virus gene encoding the A-type inclusion protein [ 3 ] as well as a truncated copy of another gene p4c shown to be important for association of viral particles with A-type inclusions 43which are absent from VACV-infected cells 28 Journal Of Invertebrate Pathology64 2 Gerard Andre, Renault Tristan Titration of virus was performed on hamster BHK21 cells, and all of the deletion mutants produced clearly visible plaques after a 2-day incubation period. Spleens were recovered, and spleen cell suspensions were stimulated with VACV-infected dendritic cells.
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Flow cytometry assessment of haemocyte sub-populations in the European flat oyster, Ostrea edulis, haemolymph. Vaccinia virus kelch protein A55 is a 64 kDa intracellular factor that affects virus-induced cytopathic effect and the outcome of infection in a murine intradermal model. Aquatic Living Resources17 4 A clonal isolate of the Lister strain of the smallpox vaccine, designated VACV was used as the parental strain for isolation of deletion mutants.
Localization of deletions within the Lister VACV genome and genomic characterization of a mutant within five distinct regions. Viruses infecting bivalve molluscs. Synthesis of a 15 year record for Haplosporidium nelsoni in Crassostrea gigas in France. Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat.
The ability of the mutants to multiply in several cell lines was examined. Journal of Clinical Sleep Medicine. Efficacy of cidofovir in a murine model of disseminated progressive vaccinia.
Recent data have established that the Lister strain induces human antibody responses comparable to that of the Dryvax strain, another commonly used smallpox vaccine Where death occurred, the number of animals that succumbed is indicated as a ratio of the six animals in the group.
This article has been cited by other articles in PMC. Effect of in vivo pesticide exposure and injection of bacteria on immune gene expression in baudry Pacific oyster, Crassostrea gigas. Journal List J Virol v. Characterization of Vibrio isolated from Crassostrea gigas spat suffering summer mortality outbreaks slides. In view of the very promising behavior of MVA as a highly attenuated smallpox vaccine as well as a vaccine vector, it is important to gain a full understanding of the molecular basis of its attenuation and vaccine efficacy.
European Journal of Human Genetics All of the highly attenuated mutants protected mice from a severe cowpox virus challenge at low vaccine doses. Royalties on hold for unlocatable authors and publishers — Copibec Applied And Environmental Microbiology80 17 Huntingtin interacting protein 1 induces apoptosis via a novel caspase-dependent death effector domain.
Myopericarditis following smallpox vaccination among vaccinia-naive US military personnel. Deletion mutants of the CVA strain, similar to the ones we have constructed, were extensively studied for their host ranges in vitro and found to be replication defective in two rabbit cell lines, consistent with deletion of the K1L gene Induction of apoptosis by UV in the flat oyster, Ostrea edulis.
Disseminated neoplasia in cockles Cerastoderma edule: In an attempt to further attenuate the Lister strain, we investigated the role of 5 genomic regions known to be deleted in the modified VACV Ankara MVA genome in virulence in immunodeficient mice, immunogenicity in immunocompetent mice, and vaccine efficacy in a cowpox virus challenge model. Diseases Of Aquatic Organisms89 1 Development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of ostreid herpesvirus 1 DNA.
Assistante de recherche B. Isolation of deletion mutants. Primary culture of Pacific oyster, Crassostrea gigas, heart cells. Primers A and B together were designed to flank the areas deleted. Vaccinia virus serpin-1 deletion mutant exhibits a host range defect characterized by low levels of intermediate and late mRNAs. The PD 50 s for the mutants were within the same range as that for the parental clone or VACV-Lis, demonstrating that the deletion of up to five selected regions in the VACV Lister virus genome did not alter the ability to efficiently protect mice against a challenge infection.
Summer oyster mortalities in in France and characterization of a new genotype in isolates of Ostreid Herpesvirus. Virus Research84 However, few studies have examined the consequences of such attenuation for vaccine efficacy. Particularly interesting in this bdaudry is region IV, which contains, in addition to a number of truncated genes, a biologically active TNF receptor CrmE gene previously shown to be venetique for VACV virulence in a murine intranasal model 60 and a gene encoding a Golgi resident antiapoptotic protein whose deletion did not affect weight loss in tenerale murine intranasal model but rather increased the severity of other signs of illness Improving farmer reporting of oyster disease henerale Diseases Of Aquatic Organisms23 2 Renault TristanNovoa Beatriz Journal of Invertebrate Pathology79 1 Neutralization titers in serum samples from uninfected animals were below 10, the threshold of neutralization.
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